Dynamics of growth of microbial cell titer of Bradyrhizobium japonicum and Sinorhizobium fredii strains during submerged cultivation in a laboratory fermenter. A.I. SOROKINA, M.V. YAKIMENKO, S.A. BEGUN

Authors

  • Арина Игоревна СОРОКИНА
  • Мария Владимировна ЯКИМЕНКО
  • Степан Алексеевич БЕГУН (Федеральный научный центр «Всероссийский научно-исследовательский институт сои», Благовещенск)

Keywords:

rhizobia, strain, B. japonicum, S. fredii, submerged cultivation, titer, carbon sources, nutrient medium

Abstract

Dynamics of growth of microbial cell titer of Bradyrhizobium japonicum and Sinorhizobium fredii strains during submerged cultivation in a laboratory fermenter. A.I. SOROKINA, M.V. YAKIMENKO, S.A. BEGUN (Federal Scientific Center “All-Russian Research Institute of Soybeans”, Blagoveshchensk).

The results of studies of the dynamics of growth of bacterial cells of Bradyrhizobium japonicum (Jordan, 1982) and Sinorhizobium fredii (Scholla, Elkan, 1984) strains in liquid culture for 24, 48, 72 hours of submerged cultivation, assimilating a wide range of carbon sources, are presented. The most technological in ability of accumulation high titer CFU/ml in conditions of submerged cultivation are the strains of B. japonicum TM-455, BM-88, TM-469, BM-91 and S. fredii OB-46, 071, TB-496, SB-38. The maximum titer of active cells of B. japonicum CM-42 and S. fredii ББ-49 strains was obtained during 72-hour cultivation in a laboratory TN-50L series fermenter, at a cultivation temperature +28 ℃, stirrer rotation at 70 rpm and pHmedium 6.73–6.77, which amounted to 8 ∙ 109 CFU/ml and 17 ∙ 109 CFU/ml, respectively.

Author Biographies

Арина Игоревна СОРОКИНА

кандидат ветеринарных наук, ведущий научный сотрудник,

Мария Владимировна ЯКИМЕНКО

кандидат биологических наук, ведущий научный сотрудник,

Степан Алексеевич БЕГУН, (Федеральный научный центр «Всероссийский научно-исследовательский институт сои», Благовещенск)

кандидат биологических наук, ведущий научный сотрудник

Published

2021-06-09

How to Cite

СОРОКИНА, А. И., ЯКИМЕНКО, М. В., & БЕГУН, С. А. (2021). Dynamics of growth of microbial cell titer of Bradyrhizobium japonicum and Sinorhizobium fredii strains during submerged cultivation in a laboratory fermenter. A.I. SOROKINA, M.V. YAKIMENKO, S.A. BEGUN. Vestnik of the Far East Branch of the Russian Academy of Sciences, (3), 92–99. Retrieved from http://vestnikdvo.ru/index.php/vestnikdvo/article/view/768

Issue

Section

Растениеводство