Surface cultivation of mycelium of shiitake mushroom (Lentinula edodes (Berk.) Pegler) on nutrient media with biostimulants. A.S. CHIBIZOVA, E.N. BARSUKOVA, G.V. GUKOV
Keywords:
artificial cultivation, Lentinula edodes, mycelium, in vitro, biostimulant, growth rate, growth coefficientAbstract
Surface cultivation of mycelium of shiitake mushroom (Lentinula edodes (Berk.) Pegler) on nutrient media with biostimulants. A.S. CHIBIZOVA, E.N. BARSUKOVA (Federal Scientific Center of Agrоbiotechnology in the Far East named after A.K. Chaika, Ussuriysk, Timiryazevsky village), G.V. GUKOV (Primorskaya State Academy of Agriculture, Ussuriysk, Timiryazevsky village).
Biological characteristics and production parameters of mycelial culture in vitro is an important link in the development of effective technologies of artificial cultivation of valuable and rare mushrooms, such as shiitake. The paper presents the results of the practical application of growth stimulants during the surface cultivation of the mycelium of the natural isolate of Shiitake (Lentinula edodes (Berk.) Pegler), isolated from its natural habitat (Ussuriysk Region). The stimulating effect of the preparations Humate+7, Epin-extra, and Zircon on the culture of L. edodes when they are added to the agarized oat nutrient has been established. The concentrations of biostimulants that contribute to the increase in the growth ratio and quality of mycelial colonies were determined: Humate+7 (50 mg/l), Epin-extra (0.5 and 0.1 ml/l), and Zircon (0.5 ml/l).