Expression and isolation optimization of recombinant analog of actinoporin Hct-A2 from sea anemone Heteractis crispa. A.P. PAVLENKO

Authors

  • Александра Павловна ПАВЛЕНКО (Тихоокеанский институт биоорганической химии им. Г.Б. Елякова ДВО РАН, Владивосток)

Keywords:

actinoporins, pore-forming toxins, sea anemones, Heteractis crispa

Abstract

Expression and isolation optimization of recombinant analog of actinoporin Hct-A2 from sea anemone Heteractis crispa. A.P. PAVLENKO (G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok).

The conditions of the expression and isolation of the recombinant analog of actinoporin Hct-A2 from sea anemone Heteractis crispa were selected. The high pressure homogenization of cells was found to increase the yield of recombinant actinoporin in a soluble form, in contrast to the ultrasonic cell destruction.

Author Biography

Александра Павловна ПАВЛЕНКО, (Тихоокеанский институт биоорганической химии им. Г.Б. Елякова ДВО РАН, Владивосток)

аспирант

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Published

2021-03-15

How to Cite

ПАВЛЕНКО, А. П. (2021). Expression and isolation optimization of recombinant analog of actinoporin Hct-A2 from sea anemone Heteractis crispa. A.P. PAVLENKO. Vestnik of the Far East Branch of the Russian Academy of Sciences, (1), 149–152. Retrieved from http://vestnikdvo.ru/index.php/vestnikdvo/article/view/712

Issue

No. 1 (2021)

Section

New scientific personnel