Features of сalibration еequations for IR scanners in determining the amino acid composition of soy proteins. S.E. NIZKII, G.A. KODIROVA, G.V. KUBANKOVA
Keywords:
IR scanner, chromatography, computer programs, calibration equations, amino acids, soybeanAbstract
Features of сalibration еequations for IR scanners in determining the amino acid composition of soy
proteins. S.E. NIZKII, G.A. KODIROVA, G.V. KUBANKOVA (All-Russian Scientific Research Institute of Soybean,
Blagoveshchensk).
17 of the 20 amino acids, included in the composition of plant proteins, are most effectively determined using
liquid chromatography. The technology of high-performance liquid chromatography is to a certain extent costly in
time, among other things because of sample preparation that makes it unsuitable for mass analysis, for example, when evaluating a breeding material. In this case, the technology based on scanning in the near infrared radiation band are the most acceptable. Despite the fact that IR scanners are able to determine a sufficiently large number of components on the basis of one calibration equation, a constant correction is required when determining the composition of amino acids and reducing it to a percentage ratio. The options for creating calibration equations for determining the amino acid composition of soybean proteins for computer programs (Nir 42, ISI), which provide the operation of IR scanners, such as NIR-4250 or FOSS NIRSystem 5000 are considered in the article. It was found that when creating calibration equations, it is most correct to set for each amino acid its mass content (g per 100 g of protein), and not the relative portion (in %).
