Usage of the method of biotechnology in the selection of buckwheat plants in the Far East. E.N. BARSUKOVA, A.G. KLYKOV, P.V. FISENKO, S.A. BOROVAYA, E.L. CHAYKINA
Keywords:
biotechnology, in vitro, Fagopyrum esculentum Moench, ISSR-analysis, rutin, heavy metal ions, selective medium, the Far EastAbstract
Usage of the method of biotechnology in the selection of buckwheat plants in the Far East. E.N. BARSUKOVA, A.G. KLYKOV, P.V. FISENKO, S.A. BOROVAYA (Federal Scientific Center of Agrobiotechnology in the Far East named after A.K. Chaika, Primorsky Krai, Ussuriysk), E.L. CHAYKINA (G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok).
The results of molecular genetic studies showed that the usage of selective media with high concentration of zinc and cuprum ions induced the most significant genetic differences among buckwheat regenerative lines. It was found in the process of microshoots cultivation on selective media with high concentration of heavy metals that the inhibiting effect of zinc and cuprum ions produced on plants was enhanced with an increase in their concentration in the nutrient medium. The rutin content in plant-regenerants of Izumrud buckwheat veriety increased by 20–22 % as compared to reference sample under conditions of ionic stress caused by high concentration of cuprum (184 mg/l) and zinc (404 mg/l). The data presented in the article confirm the potential which the usage of the biotechnology method (the selective factor of the sublethal concentrations of zinc and cuprum ions in vitro culture) in the selection of buckwheat plants for the development
of new genotypes with high level of stress resilience and flavonoids content.
