Genetic characteristics of Holstein cattle by DNA microsatellites. E.B. SHUKYUROVA, A.A. LUKASHINA, A.N. BUZKO

Authors

  • Елена Борисовна ШУКЮРОВА
  • Анастасия Алексеевна ЛУКАШИНА (Дальневосточный научно-исследовательский институт сельского хозяйства ДВО РАН, Хабаровский край, с. Восточное),
  • Андрей Николаевич БУЗЬКО (ООО «Сергеевское», Хабаровский край, с. Сергеевка)

Keywords:

cattle, Holstein breed, microsatellites, alleles, the number of effective alleles, heterozygosity

Abstract

Genetic characteristics of Holstein cattle by DNA microsatellites. E.B. SHUKYUROVA1, A.A. LUKASHINA, A.N. BUZKO2 ( 1Far Eastern Research Institute of Agriculture, FEB RAS, Khabarovsk Region, Vostochnoe village, 2“Sergeevskoe” LLC, Khabarovsk Region, Sergeevka village).

The results of genetic characteristic of 300 heads of Holstein cattle, brought from the Northern Trans-Urals at Khabarovsk Region by 15 microsatellite loci of DNA are presented in this article. It was determined that in he group of animals being understudy the number of alleles on one locus was 8.8 while effective alleles – 4.5. In general 132 alleles were revealed, four of them met often: 102 (locus CSRM 60), 117 (locus ETH 3), 117 (locus TGLA 126), 258 (locus SPS 115) with frequency from 0.538 to 0.587. The greatest level of genetic variety was characterised by locus TGLA 122, it had a maximum number of alleles – 20. The middle level of the observed and expected heterozygosity for all the studied loci was 0.700. The high level of heterozygosity testifies to the genetic variety of corresponding animal genome loci.

Author Biographies

Елена Борисовна ШУКЮРОВА

кандидат биологических наук, ведущий научный сотрудник,

Анастасия Алексеевна ЛУКАШИНА, (Дальневосточный научно-исследовательский институт сельского хозяйства ДВО РАН, Хабаровский край, с. Восточное),

младший научный сотрудник

Андрей Николаевич БУЗЬКО, (ООО «Сергеевское», Хабаровский край, с. Сергеевка)

главный зоотехник

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Published

2020-08-11

How to Cite

ШУКЮРОВА, Е. Б., ЛУКАШИНА, А. А., & БУЗЬКО, А. Н. (2020). Genetic characteristics of Holstein cattle by DNA microsatellites. E.B. SHUKYUROVA, A.A. LUKASHINA, A.N. BUZKO. Vestnik of the Far East Branch of the Russian Academy of Sciences, (4), 47–52. Retrieved from http://vestnikdvo.ru/index.php/vestnikdvo/article/view/607

Issue

No. 4 (2020)

Section

К 85-летию ДВ НИИ сельского хозяйства