The first peptide ASIC1a channel modulators from sea anemones

Authors

  • Rimma Sergeevna KALINA G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia
  • Irina Nikolaevna GLADKIKH G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia
  • Pavel Sergeevich DMITRENOK G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia
  • Elena Alexandrovna ZELEPUGA G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia
  • Margarita Mikhailovna MONASTYRNAYA G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia
  • Emma Pavlovna KOZLOVSKAYA G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia
  • Sergey Gennadievich KOSHELEV Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia
  • Sergey Alexandrovich KOZLOV Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia

Keywords:

sea anemone, peptides, modulators of ASIC channels

Abstract

Three new ASICs modulators from the sea anemone Heteractis crispa, Hcr 1b-2, Hcr 1b-3, and Hcr 1b-4, exhibit surprising activity on ASIC1a channel. Homology modeling of peptide spatial structures revealed dispositions of basic amino acid residues different from those in prototypical APETx2 toxin from Anthopleura elegantissima inhibiting ASIC3. These results support the hypothesis that Heteractis crispatoxins dock onto ASIC1a channel in different spatial orientation than their closest homolog, APETx2, do upon ASIC3 inhibition.

Author Biographies

Rimma Sergeevna KALINA, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia

Junior Research

Irina Nikolaevna GLADKIKH, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia

PhD, Senior Researcher

Pavel Sergeevich DMITRENOK, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia

PhD, Acting Director

Elena Alexandrovna ZELEPUGA, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia

PhD, Senior Researcher

Margarita Mikhailovna MONASTYRNAYA, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia

DSc, Leading Researcher

Emma Pavlovna KOZLOVSKAYA, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia

DSc, The Head of The Laboratory

Sergey Gennadievich KOSHELEV, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia

PhD, Senior Researcher

Sergey Alexandrovich KOZLOV, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia

DSc, The Head of The Laboratory

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Published

2019-06-13

How to Cite

KALINA, R. S., GLADKIKH, I. N., DMITRENOK, P. S., ZELEPUGA, E. A., MONASTYRNAYA, M. M., KOZLOVSKAYA, E. P., KOSHELEV, S. G., & KOZLOV, S. A. (2019). The first peptide ASIC1a channel modulators from sea anemones. Vestnik of the Far East Branch of the Russian Academy of Sciences, (6s), 41–43. Retrieved from http://vestnikdvo.ru/index.php/vestnikdvo/article/view/224

Issue

No. 6s (2018): 6 Supplement

Section

Статьи