Use of modified Zarrouk medium for recultivation of cyanobacteria Arthrospira platensis and Spirulina subsalsa after cryopreservation.

Abstract

The aim of the study was to estimate the increase of biomass and synthesis products content of cyanobacteria Arthrospira platensis and Spirulina subsalsa after cryopreservation. Cyanobacteria have been recultivated after thawing in recycled Zarrouk medium with the additions of whey retentate. With these culture conditions, the increase in biomass, carbohydrates, proteins, lipids and phenolic compounds in S. subsalsa and A. platensis after cryopreservation has been determined. The recycled Zarrouk medium with the addition of 2.0 % of modified or original whey retentate has been appropriate for use to cyanobacteria recultivation after their cryopreservation. Investigation results obtained can be used for recovery after cryopreservation of different cyanobacteria cultures.