INDEL-typing of strains of parahemolytic vibrions in vitro and in silico

Abstract

The article presents the results of studying the distribution of Vibrio parahaemolyticus INDEL genotypes in the world and in Russia in vitro and in silico using the previously developed INDEL typing method based on the distribution of alleles of six loci (Vp967, Vp08, Vp619, Vp2256, VpA472, and Vp506). Among 2105 strains of V. parahaemolyticus taken in the study, 30 unique INDEL genotypes were identified, seven of which were identified as major. At the same time, geographical attachment between the genotype of parahaemolytic vibrions and the place of their isolation was revealed. The circulation of 17 INDEL genotypes was detected on the territory of the Russian Federation, the predominant of which was D2, found only in the Russian Federation. As a result, it was found that the proposed INDEL-typing method is a useful tool in conducting epidemiological investigations, which makes it possible to identify phylogenetic relationships between different isolates, which in turn makes it possible to draw conclusions about the sources of splinters of the pathogen to a specific territory.