Chromatographic determination of fluorecscein in natural waters and technological solutions

Abstract

A method for the chromatographic determination of residual concentrations of fluorescein in natural waters and technological solutions with a detection limit of 1ng/l has been developed. To isolate fluorescein from waters, solid-phase extraction on Diapak C16 cartridges is used, chromatographic separation is carried out on a Shodex Asahipak ODP-50 column using tetrabutylammonium ion-pair reagent and fluorimetric detection. The method was tested in determining the residual concentration of fluorescein in the industrial monitoring program at an oil producing facility. The possibility of using fluorescein to detect low-level leaks from nuclear power plants (NPP) has been studied. It has been shown that fluorescein is sufficiently thermally stable and can be used as an indicator of the leakage of the coolant solution of the secondary circuit of a NPP with a VVER-1000 reactor.