Condition optimization to obtain recombinant mammalian α-amylase inhibitor, magnificamide, sea anemone Heteractis magnifica

Authors

  • Daria V. Popkova G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia

Keywords:

diabetes, α-amylase inhibitors, sea anemones, Heteractis magnifica

Abstract

The obtaining conditions of recombinant inhibitor of mammalian α-amylase, magnificamide, sea anemone Heteractis magnifica were optimized. The fusion protein refolding procedure was revealed to lead to a significant yield increase of the recombinant peptide with correct spatial structure in comparison with the peptide isolation without the refolding.

Author Biography

Daria V. Popkova, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia

Laboratory Assistant

https://orcid.org/0000-0002-7463-5653

Downloads

Published

2022-12-07

How to Cite

Popkova Д. В. (2022). Condition optimization to obtain recombinant mammalian α-amylase inhibitor, magnificamide, sea anemone Heteractis magnifica. Vestnik of the Far East Branch of the Russian Academy of Sciences, (6), 143–148. Retrieved from http://vestnikdvo.ru/index.php/vestnikdvo/article/view/1032

Issue

No. 6 (2022)

Section

New scientific personnel