Co-cultivation of marine micromycetes fungi is a promising way of obtaining new bioactive secondary metabolites. Sh.Sh. AFIYATULLOV, O.I. ZHURAVLEVA

Abstract

Five new diketopiperazine alkaloids of the notoamide family, the new diphenyl ether diorcinol J and the new
chromene metabolite oxyrapentin L were isolated by co-cultivation of Aspergillus sulphureus and Isaria felina fungi.
Structures of the isolated compounds were determined based on two-dimensional NMR spectroscopy and high-resolution mass spectrometry. The absolute configurations of alkaloids were established on the basis of calculations of the electron circular dichroism (ECD) spectra in the framework of the non-stationary density functional theory (TD-DFT) and comparison of CD spectra with published data. The cytotoxic activity of the isolated compounds was studied.