The effect of pH on the chaperone activity of Skp from Yersinia pseudotuberculosis

Аннотация

The aim of this study was to evaluate the effect of pH on the behavior Skp of Yersinia pseudotuberculosis in solution and its manifestation of chaperone activity. Commercial samples of Fc- and Fab-fragments of human IgG were used as protein substrates for binding to Skp chaperone. The aggregation kinetics of rSkp, Fc- and Fab-fragments and protein substrates in the presence of a chaperone in acidic, neutral, and basic solutions was studied using the dynamic light scattering method. The obtained results demonstrate the pH-dependent character of the chaperone activities of rSkp. The most stable low-molecular complexes (RH up to 10 nm) between protein substrates and chaperone are formed atacidic pH values of the medium. In the case of alkalinization of the reaction medium, the chaperone activity of rSkp decreases, chaperone forms weakly stable complexes with Fc- and Fab-fragments of human IgG,which do not exclude further self-association and aggregation of protein substrates